mouse anti human b7h3 antibody  (R&D Systems)

Journal: Frontiers in Oncology

Article Title: Association of B7H3 with HIF-1α nuclear expression indicates poor prognosis and therapeutic potential in gastric cancer

doi: 10.3389/fonc.2026.1744341

Figure Lengend Snippet: (A) Analysis of B7H3 and HIF-1α gene expression levels in gastric cancer (GC) and adjacent noncancerous tissues. (B) Analysis of the correlation between B7H3 and HIF-1α in gastric adenocarcinoma using the GEPIA database. (C) Analysis of the correlation between B7H3 and HIF-1α in adjacent noncancerous tissues using the GEPIA database. (D) Survival curve of patients with gastric cancer based on B7H3 expression. (E) Survival curve of patients with gastric cancer based on HIF-1α expression. (F) Survival curve of patients with gastric cancer based on combined B7H3 and HIF-1α expression. The red line indicates the high expression group, the blue line indicates the low expression group, and the yellow line indicates the other groups. **** indicates P < 0.001.

Article Snippet: Subsequently, the sections were incubated overnight at 4°C with mouse anti-human B7H3 antibody (R&D Systems, MN, USA, #AF1027, 1:200) or rabbit anti-human HIF-1α antibody(R&D Systems, MN, USA, #MAB19352,Clone #2443C,1:200).

Techniques: Gene Expression, Expressing

Journal: Frontiers in Oncology

Article Title: Association of B7H3 with HIF-1α nuclear expression indicates poor prognosis and therapeutic potential in gastric cancer

doi: 10.3389/fonc.2026.1744341

Figure Lengend Snippet: (A) Representative immunohistochemical (IHC) images of B7H3 and HIF-1α expression in gastric cancer tissues from 268 clinical patients with gastric cancer. (B) Representative IHC images of B7H3 and HIF-1α nuclear expression in gastric cancer tissues from 268 clinical patients with gastric cancer. Scale bars: 100 μm; 25 μm. (C) resentative multiplex fluorescence immunohistochemistry (mIHC) images of high B7H3 expression and HIF-1α nuclear expression in gastric cancer tissues. Scale bar: 100 μm.

Article Snippet: Subsequently, the sections were incubated overnight at 4°C with mouse anti-human B7H3 antibody (R&D Systems, MN, USA, #AF1027, 1:200) or rabbit anti-human HIF-1α antibody(R&D Systems, MN, USA, #MAB19352,Clone #2443C,1:200).

Techniques: Immunohistochemical staining, Expressing, Multiplex Assay, Fluorescence, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: Association of B7H3 with HIF-1α nuclear expression indicates poor prognosis and therapeutic potential in gastric cancer

doi: 10.3389/fonc.2026.1744341

Figure Lengend Snippet: (A) Survival curve of gastric cancer patients based on B7H3 expression levels. (B) Survival curve of patients with gastric cancer based on HIF-1α expression levels. (C) Survival curve of patients with gastric cancer based on whether HIF-1α was expressed in the nucleus. (D) Survival curve of patients with gastric cancer based on the co-expression of B7H3 and HIF-1α. (E) Survival curve of patients with gastric cancer based on the co-expression of B7H3 and nuclear HIF-1α.

Article Snippet: Subsequently, the sections were incubated overnight at 4°C with mouse anti-human B7H3 antibody (R&D Systems, MN, USA, #AF1027, 1:200) or rabbit anti-human HIF-1α antibody(R&D Systems, MN, USA, #MAB19352,Clone #2443C,1:200).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Association of B7H3 with HIF-1α nuclear expression indicates poor prognosis and therapeutic potential in gastric cancer

doi: 10.3389/fonc.2026.1744341

Figure Lengend Snippet: (A) HIF-1α protein expression in HGC-27 cells under gradient concentrations of cobalt chloride induction. (B) B7H3 protein expression in HGC-27 cells under gradient concentrations of cobalt chloride (40 μmol/L, 200 μmol/L, 1000 μmol/L) induction. (C) HIF-1α protein expression in HGC-27 cells with overexpressed or knocked-down B7H3 under hypoxic and normal conditions. (D) HIF-1α expression in nuclear and cytoplasmic proteins of HGC-27 cells with overexpressed or knocked-down B7H3 under cobalt chloride induction. (E) Fluorescence immunohistochemistry of HGC-27 cells with B7H3 knockdown (SHB),B7H3 overexpression (LVB) and control group (SHN/LVN). Blue fluorescence represents DAPI, and red fluorescence represents HIF-1α. Scale bar: 10 μm.

Article Snippet: Subsequently, the sections were incubated overnight at 4°C with mouse anti-human B7H3 antibody (R&D Systems, MN, USA, #AF1027, 1:200) or rabbit anti-human HIF-1α antibody(R&D Systems, MN, USA, #MAB19352,Clone #2443C,1:200).

Techniques: Expressing, Fluorescence, Immunohistochemistry, Knockdown, Over Expression, Control